MEK Inhibition Targets Cancer Stem Cells and Impedes Migration of Pancreatic Cancer Cells In Vitro and In Vivo
Effects of MEK inhibition on TGFβ-induced EMT. (a) Experimental overview for TGFβ and PD0325901 co–treatment. (b) Western blot analysis of key proteins involved in EMT with treatment in vitro. Gapdh was used as a loading control. (c) Percentage of RFP+YFP+ cells under treatment as indicated and representative cytometry blots. (d) Immunofluorescence micrographs of E-cadherin expression with treatment as indicated. for all experiments, for Western blots. vs. control, vs. TGFβ.