Injectable Hydrogel Combined with Nucleus Pulposus-Derived Mesenchymal Stem Cells for the Treatment of Degenerative Intervertebral Disc in Rats
Identification of nucleus pulposus-derived mesenchymal stem cells (NPMSC). (a) Isolated primary cells presented with a long spindle shape and showed colony growth; the passaged cells were spindle-shaped. (b) NPMSC had higher expressions (>98%) of MSC surface markers CD73, CD90, and CD105 and lower expressions (<2%) of hematopoietic stem cell surface markers CD34 and CD45 and higher expressions (>80%) of Tie2. (c) NPMSC was positive for Alizarin red, Oil Red O, and Alcian blue staining after induced differentiation. White scale .