Injectable Hydrogel Combined with Nucleus Pulposus-Derived Mesenchymal Stem Cells for the Treatment of Degenerative Intervertebral Disc in Rats
Cell proliferation and differentiation. (a) Representative images of DAPI staining for 2D (control group) and 3D (hydrogel group) cultured NPMSC at 1, 4, and 8 days after cultivation. (b) Representative images of Edu staining for 2D (control group) and 3D (hydrogel group) cultured NPMSC at 8 days after cultivation. (c) Investigation of growth rate and Edu-positive rate of NPMSC culture for 8 days in the control group and hydrogel group. (d) Relative protein levels of collagen type II and aggrecan in the control group and hydrogel group. Data represent ; the error bars represent the standard deviation of measurements for 3 separate samples (); compared with the hydrogel group. White scale .
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