Designing Microfluidic Devices to Sort Haematopoietic Stem Cells Based on Their Mechanical Properties

Figure 4

Separation efficiency of HSCs using spiral Device D. (a) At both flow rates (5 ml/h and 7.5 ml/h), inner outlet HSCs had significantly lower NRS values and were thus more deformable than outer outlet HSCs. (b) Mean diameter of inner outlet HSCs was significantly smaller only at the higher flow rate. (c) At the lower flow rate (5 ml/h), NRS distribution of inner outlet HSCs was wider () than at the (d) higher flow rate (10 ml/h; ). Hence, this device performed more sensitive sorting at 7.5 ml/h. as determined using a paired Student -test.