Stem Cells International / 2019 / Article / Tab 1 / Research Article
Preconditioning of Human Dental Pulp Stem Cells with Leukocyte- and Platelet-Rich Fibrin-Derived Factors Does Not Enhance Their Neuroregenerative Effect Table 1 Primary and secondary antibodies for immunocytochemical analysis.
Marker Species Clone, Cat no. Dilution Label Company Primary antibodies A2B5 MM MAB312R 1/200 Millipore, Billerica, MA, USA BLBP RP ABN14 1/250 Millipore β -III tubulinRP ab18207 1/50 Abcam, Cambridge, UK β -III tubulinMM 2G10 1/2000 Sigma-Aldrich CD45 RP Ab63390 1/100 Abcam GFAP MM Clone G-A-5 1/400 Sigma-Aldrich NCAM RP Ab5032 1/100 Millipore NeuN MM A60, MAB377 1/100 Millipore Sca-1 GP AF1226 1/50 R&D, Minneapolis, MN, USA Sox2 RP Ab97959 1/1000 Abcam Secondary antibodies DAM DP IgG A31570 1/500 Alexa Fluor 555 Invitrogen, Carlsbad, CA, USA DAR DP IgG A21206 1/500 Alexa Fluor 488 Invitrogen DAG DP IgG A11055 1/500 Alexa Fluor 488 Invitrogen
BLBP: brain lipid-binding protein; GFAP: glial fibrillary acidic protein; NCAM: neural cell adhesion molecule; NeuN: neuronal nuclei; Sca-1: stem cell antigen-1; MM: mouse monoclonal antibody; RP: rabbit polyclonal antibody; GP: goat polyclonal; DP: donkey polyclonal; DAM: donkey anti-mouse; DAR: donkey anti-rabbit; DAG: donkey anti-goat.
