Protection of the Peritoneal Membrane by Peritoneal Dialysis Effluent-Derived Mesenchymal Stromal Cells in a Rat Model of Chronic Peritoneal Dialysis

Figure 1

Isolation and culture of pMSCs. Peritoneal cells from PD effluents were grown in the xeno-free medium in plastic petri dishes, followed by medium change once every 2-3 days. Data represented a typical microscopic image of different stages (passages) of cell cultures. P0: seeding peritoneal cells after removal of nonadherent cells by changing the culture medium at the beginning of pMSC culture. Red “M”: macrophages. P1: cells on passage 1 of culture. Yellow arrows: MSC colonies. P2 or P3: fibroblast-like cells on passage 2 or 3 of culture. Red arrows: “cobblestone”-like mesothelial cells. P4: a homogenous monolayer of spindle-shaped pMSCs in culture. Scale bar: 100 μm.