Research Article

Protection of the Peritoneal Membrane by Peritoneal Dialysis Effluent-Derived Mesenchymal Stromal Cells in a Rat Model of Chronic Peritoneal Dialysis

Figure 5

Incubation with pMSC-CM inhibits hypertonic peritoneal dialysis solution- (PDS-) induced apoptosis in cultured HPMCs. HPMCs ( cells/well) were grown in 24-well plates overnight and were briefly exposed to Dianeal (4.25% dextrose), followed by being cultured in the xeno-free medium in the absence or presence of 50% (/) of pMSC-CM for 24 h or 48 h. Cell apoptosis was determined by flow cytometric analysis with Annexin-V-PE and 7-AAD staining. (a) A typical dot plot showing the percentage of Annexin-V-PE- and/or 7-AAD-positive staining of cell populations in each group. (b) The sum of Annexin V-stained cell populations as total apoptosis in each group. Data are presented as of each group (medium: , PDS: , and PDS+50%: ) and were statistically analyzed by using the two-tailed -test. (PDS vs. medium), (PDS+50% vs. PDS), (PDS vs. medium), and (PDS+50% vs. PDS).
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