Protection of the Peritoneal Membrane by Peritoneal Dialysis Effluent-Derived Mesenchymal Stromal Cells in a Rat Model of Chronic Peritoneal Dialysis
Table 1
Inactivation of inflammatory responses of macrophages by pMSC-CM.
Affected gene transcripts
Fold changes by PMA/LPS (vs. control)
value (PMA/LPS vs. control, )
Fold changes by pMSC-CM (vs. PMA/LPS)
value (PMA/LPS+50% vs. PMA/LPS, )
CXCL8
535.95
0.009573
-9.14
0.083365
IL1B
500.8
0.048456
-6.25
0.123176
CXCL6
242.74
0.001276
-10.08
0.032372
CXCL3
190.02
0.038563
-2.32
0.133338
IL1RN
177.15
0.004863
-4.02
0.037209
NOS2
11.17
0.007915
-9.35
0.008181
RIPK2
6.02
0.013444
-5.35
0.058044
BCL6
5.27
0.029664
-2.39
0.085688
CCL5
3.8
0.065136
-8.77
0.017685
ITGB2
2.97
0.036245
-2.54
0.056093
NR3C1
2.45
0.234157
-7.81
0.043945
MYD88
2.82
0.092337
-4.90
0.058324
TNF
1.98
0.052401
-1.46
0.140847
CCR1
-1.27
0.428263
1.74
0.026517
CCR4
1.18
0.48383
1.92
0.01117
CXCL9
-1.67
0.138019
1.97
0.000092
CXCR1
-1.11
0.662785
1.60
0.035095
IL10
-1.38
0.309707
1.93
0.015066
IL23A
1.12
0.851924
1.60
0.016877
IL6R
-1.36
0.179679
1.65
0.004205
IL9
-1.25
0.522482
1.93
0.024161
KNG1
-1.25
0.320858
1.80
0.01354
SELE
-1.2
0.477778
1.86
0.005707
TLR3
-1.26
0.363668
2.17
0.008944
TLR5
-1.38
0.298214
1.86
0.036134
The regulatory effects (both upregulation and downregulation) of pMSC-CM on the expression of inflammation-related gene transcripts in PMA/LPS-activated THP1 cells were examined by using the PCR array. The fold change of each transcript level in the experimental group as compared to the control group (PMA/LPS vs. control or PMA/LPS+50% pMSC-CM vs. PMA/LPS) was presented as mean of three separate RNA samples (). A negative number represented downregulation.
