Does the Harvesting Site Influence the Osteogenic Potential of Mesenchymal Stem Cells?

Figure 3

Chondrogenic differentiation in suspension condition. Cells were induced in chondrogenic medium in suspension condition to form pellets up to 21 days and then analyzed. (a) Proliferation of the cells determined by MTT assay. Absorbance of the stained cells was measured at 570 nm and 670 nm (reference wavelength). Results are the value of at least three experiments performed in triplicate from at least three independent cell cultures. (b) Photos of toluidine blue O (TB) stain and immunohistochemistry for collagen type I (Col I) and collagen type II (Col II). Toluidine blue stains the matrix from light blue to violet and dyes the nuclei to dark blue. The white dash line covers the most violet zone on the photo showing the coloration of the more intensified cartilage matrix. The insets better illustrate cartilaginous matrix where chondrocytes are trapped in the lacunae. The brown color indicates the expression of collagen type I and collagen type II. Scale bar: insets 5 μm, others 25 μm. (c) qPCR results. Expression of the cartilage markers collagen type I, collagen type II, and Sox9. Data are presented as value (, , , ).

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