Does the Harvesting Site Influence the Osteogenic Potential of Mesenchymal Stem Cells?

Table 1

Primer sequences for qPCR (F/R: forward/reverse).

	Human gene	Sequence (5-3)	Product size (bp)
Housekeeping genes	18S rRNA	F: GTAACCCGTTGAACCCCATT	151 [21]
		R: CCATCCAATCGGTAGTAGCG
	GAPDH (NM_001289745.2)	F: ATGGGGAAGGTGAAGGTCG	70
		R: TAAAAGCAGCCCTGGTGACC
Adipogenic differentiation	PPARG (NM_001354667)	F: GGCTTCATGACAAGGGAGTTTC	74
		R: AACTCAAACTTGGGCTCCATAAAG
	LPL (NM_000237.2)	F:GAGGTACTTTTCAGCCAGGATGTAAC	82
		R: AGCTGGTCCACATCTCCAAGTC
Chondrogenic differentiation	COL1A1 (NM_000088.3)	F: CAGCCGCTTCACCTACAGC	83
		R: TTTTGTATTCAATCACTGTCTTGCC
	COL2A1 (NM_001844.5)	F: GGCAATAGCAGGTTCACGTACA	79
		R: CGATAACAGTCTTGCCCCACTT
	Sox9 (NM_000346.3)	F: AGTACCCGCACTTGCACAA	68
		R: CTCGTTCAGAAGTCTCCAGAGCTT
Osteogenic differentiation	BGLAP (osteocalcin) (NM_199173.5)	F: CGCCTGGGTCTCTTCACTAC	143
		R: CTCACACTCCTCGCCCTATT
	SPP1 (osteopontin) (NM_001251830.1)	F: CTCAGGCCAGTTGCAGCC	81
		R: CAAAAGCAAATCACTGCAATTCTC
	ALP (NM_000478.6)	F: ACCACCACGAGAGTGAACCA	79
		R: CGTTGTCTGAGTACCAGTCCC

18S rRNA: 18S ribosomal RNA; GAPDH: glyceraldehyde-3-phosphate dehydrogenase; PPARG: peroxisome proliferator-activated receptor gamma; LPL: lipoprotein lipase; COL1A1: collagen type I alpha 1 chain; COL2A1: collagen type II alpha 1 chain; BGLAP: bone gamma-carboxyglutamate protein; SPP1: secreted phosphoprotein 1; ALP: alkaline phosphatase.