Isolation, Characterization, and Safety Evaluation of Human Skin-Derived Precursors from an Adherent Monolayer Culture System

Figure 1

tSKP isolation protocols. Skin samples were cut into pieces with epidermis removed, followed by enzymatic digestion and mechanical dissociation into single cells. Dermal cells were seeded into a FB-adherent medium containing 10% FBS; mature FBs were then transferred into a SKP-proliferating medium. After an average of 7 days, mature spheres could be observed.