Isolation, Characterization, and Safety Evaluation of Human Skin-Derived Precursors from an Adherent Monolayer Culture System

Figure 2

tSKPs were generated from primary adherent FB cultures. (a) Phase contrast images of monolayer adherent FBs at P1, P5, and P10 (left panel) and tSKPs derived from the left FB culture (right panel). Note the characteristic morphology, which was very similar to primary SKPs. Typical SKP spheres had been successfully generated from P1, P5, and P10 FBs in this study. (b) Formation of tSKP spheres derived from P3 FBs from day 1 to 14 in culture. A typical SKP sphere appeared after approximately 7 days. Inset in day 12 shows the typical morphology of a mature sphere. Scale bars: 100 μm. (c) Measurement of tSKP number from FBs at different passages at day 7. (d) Measurement of a tSKP size from FBs at different passages at day 7.

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