Table 1: Growth parameters of adult NCSCs cultures originated from human hair follicles and skin dermis.

Cell lineP0P1P2P3CFU frequency at P3 (%)
Cell culture vesselCell yield (×106)Cell culture vesselCell yield (×106)PDNPDT (hours)Cell culture vesselCell yield (×106)PDNPDT (hours)Cell culture vesselCell yield (×106)PDNPDT (hours)

HF NCSCs 12 × Petri dish, ø 35 mm0.183T25 flask1.3555.7429.275-layer multiflask44.3945.6429.795-layer multiflask42.1555.5730.1622.4
HF NCSCs 22 × Petri dish, ø 35 mm0.142T25 flask0.935.2032.315-layer multiflask39.4625.4630.775-layer multiflask41.6475.5530.2726.8
HF NCSCs 32 × Petri dish, ø 35 mm0.225T25 flask1.2655.6429.795-layer multiflask42.7275.5930.055-layer multiflask40.0325.530.5520.5
HF NCSCs 42 × Petri dish, ø 35 mm0.114T25 flask1.4285.8128.925-layer multiflask51.2155.8528.725-layer multiflask44.5095.6529.7331.7
HF NCSCs 52 × Petri dish, ø 35 mm0.163T25 flask1.1735.5330.385-layer multiflask47.6055.7529.225-layer multiflask43.5845.6229.8928.2

Mean0.1651.2305.58430.13245.0815.65829.70942.3855.57830.12125.92
SD0.0420.1930.2391.3364.5160.1500.7861.7380.0590.3184.50
SD NCSCs 1T25 flask
3D gel
2.1925-layer multiflask56.2845.9928.055-layer multiflask58.5446.0427.815-layer multiflask53.0575.9028.4731.5
SD NCSCs 2T25 flask
3D gel
2.5175-layer multiflask59.3186.0627.725-layer multiflask62.056.1327.415-layer multiflask60.8276.1027.5433.2
SD NCSCs 3T25 flask
3D gel
3.2075-layer multiflask66.4366.2227.015-layer multiflask64.7196.1927.145-layer multiflask62.5866.1427.3638.4
SD NCSCs 4T25 flask
3D gel
1.4585-layer multiflask48.3755.7729.125-layer multiflask54.8945.9528.245-layer multiflask52.4555.8828.5730.9
SD NCSCs 5T25 flask
3D gel
2.3725-layer multiflask62.0856.1327.415-layer multiflask55.3255.9628.195-layer multiflask61.0956.1027.5436.5

Mean2.34958.5006.03427.86059.1066.05427.75758.0046.02427.89834.10
SD0.6296.7810.1700.8004.2580.1050.4804.8420.1240.5763.24