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Stem Cells International

Stem Cells International / 2019 / Article / Tab 1

Research Article

Comparative Analysis of Biological Properties of Large-Scale Expanded Adult Neural Crest-Derived Stem Cells Isolated from Human Hair Follicle and Skin Dermis

Table 1

Growth parameters of adult NCSCs cultures originated from human hair follicles and skin dermis.
Cell lineP0P1P2P3CFU frequency at P3 (%)
Cell culture vesselCell yield (×106)Cell culture vesselCell yield (×106)PDNPDT (hours)Cell culture vesselCell yield (×106)PDNPDT (hours)Cell culture vesselCell yield (×106)PDNPDT (hours)
HF NCSCs 12 × Petri dish, ø 35 mm0.183T25 flask1.3555.7429.275-layer multiflask44.3945.6429.795-layer multiflask42.1555.5730.1622.4
HF NCSCs 22 × Petri dish, ø 35 mm0.142T25 flask0.935.2032.315-layer multiflask39.4625.4630.775-layer multiflask41.6475.5530.2726.8
HF NCSCs 32 × Petri dish, ø 35 mm0.225T25 flask1.2655.6429.795-layer multiflask42.7275.5930.055-layer multiflask40.0325.530.5520.5
HF NCSCs 42 × Petri dish, ø 35 mm0.114T25 flask1.4285.8128.925-layer multiflask51.2155.8528.725-layer multiflask44.5095.6529.7331.7
HF NCSCs 52 × Petri dish, ø 35 mm0.163T25 flask1.1735.5330.385-layer multiflask47.6055.7529.225-layer multiflask43.5845.6229.8928.2
Mean 0.165 1.2305.58430.132 45.0815.65829.709 42.3855.57830.12125.92
SD 0.042 0.1930.2391.336 4.5160.1500.786 1.7380.0590.3184.50
SD NCSCs 1T25 flask
3D gel		2.1925-layer multiflask56.2845.9928.055-layer multiflask58.5446.0427.815-layer multiflask53.0575.9028.4731.5
SD NCSCs 2T25 flask
3D gel		2.5175-layer multiflask59.3186.0627.725-layer multiflask62.056.1327.415-layer multiflask60.8276.1027.5433.2
SD NCSCs 3T25 flask
3D gel		3.2075-layer multiflask66.4366.2227.015-layer multiflask64.7196.1927.145-layer multiflask62.5866.1427.3638.4
SD NCSCs 4T25 flask
3D gel		1.4585-layer multiflask48.3755.7729.125-layer multiflask54.8945.9528.245-layer multiflask52.4555.8828.5730.9
SD NCSCs 5T25 flask
3D gel		2.3725-layer multiflask62.0856.1327.415-layer multiflask55.3255.9628.195-layer multiflask61.0956.1027.5436.5
Mean 2.349 58.5006.03427.860 59.1066.05427.757 58.0046.02427.89834.10
SD 0.629 6.7810.1700.800 4.2580.1050.480 4.8420.1240.5763.24
      

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