Comparative Analysis of Biological Properties of Large-Scale Expanded Adult Neural Crest-Derived Stem Cells Isolated from Human Hair Follicle and Skin Dermis

Table 2

Phenotype characterization of adult NCSC cultures originated from human hair follicles and skin dermis at passage 3; flow cytometry.

Cell line	Antigens
	Sox10	CD271	Nestin	CD73	CD90	CD105	CD140a	CD140b	CD146	CD166	CD349	CD34	CD45	CD56	CD117	HLA-DR
HF NCSCs 1	91.0	79.1	91.2	98.9	98.3	97.2	89.5	96.9	91.6	98.3	91.0	1.3	0.3	0.3	1.1	0.8
HF NCSCs 2	96.4	82.5	97.2	99.2	98.1	94.3	82.3	92.5	93.2	95.4	95.7	0.9	0.5	0.8	0.9	0.6
HF NCSCs 3	92.8	80.8	93.4	97.4	97.8	90.8	72.4	83.7	82.7	91.8	91.6	0.2	0.6	0.9	1.3	0.7
HF NCSCs 4	95.4	91.4	92.2	99.5	99.2	93.4	90.7	93.1	92.4	94.7	96.2	0.7	0.2	0.7	0.7	0.8
HF NCSCs 5	91.2	74.3	94.1	96.8	97.4	87.2	78.7	84.6	83.6	92.5	90.7	1.1	1.0	0.8	1.2	0.5
Mean	93.36	81.62	93.62	98.36	98.16	92.58	82.72	90.16	88.70	94.54	93.04	0.84	0.52	0.70	1.04	0.68
SD	2.45	6.27	2.29	1.19	0.67	3.78	7.62	5.75	5.11	2.58	2.68	0.42	0.31	0.23	0.24	0.13
SD NCSCs 1	98.1	93.8	95.7	99.5	98.7	99.6	99.3	98.8	99.3	99.6	98.8	0.0	0.1	0.2	0.8	0.9
SD NCSCs 2	97.4	92.1	98.2	98.8	97.9	95.6	90.4	93.7	95.1	95.8	97.2	0.3	0.0	0.3	1.2	0.2
SD NCSCs 3	98.5	95.7	99.0	99.3	97.3	97.8	94.8	96.2	98.6	96.1	98.3	0.1	0.0	0.8	0.4	0.4
SD NCSCs 4	94.3	85.7	93.7	98.2	98.2	96.2	92.5	95.4	92.6	93.4	93.9	0.2	0.4	0.2	0.5	0.7
SD NCSCs 5	96.9	91.4	98.5	99.1	98.5	99.1	97.1	98.3	97.4	99.2	97.4	0.3	0.2	0.5	0.6	0.3
Mean	97.04	91.74	97.02	98.98	98.12	97.66	94.82	96.48	96.60	96.82	97.12	0.18	0.14	0.40	0.70	0.50
SD	1.65	3.76	2.25	0.51	0.55	1.75	3.54	2.10	2.75	2.58	1.91	0.13	0.17	0.25	0.32	0.29