The Influence of Negative Pressure and of the Harvesting Site on the Characteristics of Human Adipose Tissue-Derived Stromal Cells from Lipoaspirates

Scheme 1

Scheme of the experiment. Sites in the abdomen, the inner thigh, and the outer thigh where liposuction at low negative pressure (-200 mmHg) and at high negative pressure (-700 mmHg) was performed. After the cell isolation, the initial yields of attached cells were counted. In subsequent passages, the number, viability, diameter, doubling time, mitochondrial activity (all in passage 1), and CD surface markers (passage 2) of isolated ADSCs were evaluated.