Flufenamic Acid Inhibits Adipogenic Differentiation of Mesenchymal Stem Cells by Antagonizing the PI3K/AKT Signaling Pathway

Figure 1

FFA inhibits adipogenic differentiation of hASCs in vitro. (a, b) Oil red O staining (a) and quantification (b) indicated that FFA at 12.5, 25, 50, 100, and 200 μM inhibited lipid droplet formation in hASCs. Human ASCs were treated with PM, AM, or AM+FFA at 12.5, 25, 50, 100, or 200 μM for 21 days before Oil red O staining and quantification. . (c, d) FFA at 12.5, 25, 50, 100, and 200 μM inhibited the expression of PPARG (c) and CEBPA (d) in hASCs, as determined by qRT-PCR. All data are shown as the , . , , and compared with AM. PM: proliferation media; AM: adipogenic media; FFA: flufenamic acid; hASC: human adipose-derived stem cell; PPARG: peroxisome proliferator activated receptor gamma; CEBPA: CCAAT enhancer binding protein alpha; qRT-PCR: quantitative real-time reverse transcription PCR.

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