Stem Cells International / 2020 / Article / Fig 4

Research Article

Succinate Supplement Elicited “Pseudohypoxia” Condition to Promote Proliferation, Migration, and Osteogenesis of Periodontal Ligament Cells

Figure 4

Succinate supplement elicited intracellular succinate accumulation, pseudohypoxia condition, and glycolysis. The intracellular succinate in cell lysates and extracellular succinate in supernatants were assessed () (a, b). Changes in PHD2 and HIF-1α at 2 h after succinate treatment were measured by western blot (c). The mRNA and protein of genes involved in the OxPhos and glycolysis were analyzed at 4 h by qPCR and at 24 h by western blot, respectively (d, e). SDH activity was measured by ELISA after 6 h stimulation () (f). Blots were representative of 3 independent tests. , relative to control; relative to control.

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