Placental Mesenchymal Stromal Cells (PMSCs) and PMSC-Derived Extracellular Vesicles (PMSC-EVs) Attenuated Renal Fibrosis in Rats with Unilateral Ureteral Obstruction (UUO) by Regulating CD4⁺ T Cell Polarization

Figure 3

Effects of PMSCs injection on regulating CD4⁺CD25⁺Foxp3 T cells and IL17⁺IFN^-T cells of UUO rats in vivo. (a) IHC staining of Foxp3 in kidney sections from Sham, Saline, and PMSC group at days 3, 7, and 14 after UUO, respectively. Representative pictures are acquired. Scale bar: 50 μm. Foxp3 positive percentage was quantitated by measuring positive area from representative images and analyzed by image J software. compared with Saline group; compared with Saline group; ^# compared with Sham group. (b) The percentage of CD4⁺CD25⁺ T cells in CD4⁺ T cells extracted from PMNCs as well as the percentage of IL-17⁺IFN^-γ T cell in CD4⁺ T cells extracted from rat spleen homogenate was analyzed by FACs, respectively. ^# indicates VS. sham. compared with Saline group; ^# compared with Sham group.

(a)

(b)