Placental Mesenchymal Stromal Cells (PMSCs) and PMSC-Derived Extracellular Vesicles (PMSC-EVs) Attenuated Renal Fibrosis in Rats with Unilateral Ureteral Obstruction (UUO) by Regulating CD4⁺ T Cell Polarization

Figure 6

PMSC-EVs treatment attenuate tubulointerstitial fibrosis and increase Foxp3 expression by downregulating IL17 expression in vivo. (a) IHC for detecting α-SMA and COL-I expression in rat renal tissues from the UUO group and UUO+EV treatment group. Scale bar: 50 μm. (b) IHC for Foxp3 and IL-17 in rat renal tissue sections 7 days after UUO with or without PMSC-EVs treatment. Scale bar: 50 μm. (c) Fibrosis area and Foxp3 and IL17 positive cells in representative images from (a) and (b) were analyzed by Image J software. Error bars are ±SD. compared with the UUO group; compared with the UUO group. compared with UUO group. (d) IF for identification of Foxp3⁺ T cells infiltration in renal tissue after UUO 7 days with or without PMSC-EVs treatment. Scale bar: 50 μm. (e) Representative IF staining pictures of IL-17⁺ T cells in the UUO group and UUO+EV group. Scale bar: 50 μm.

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