hCTLA4-Gene-Modified Human Bone Marrow-Derived Mesenchymal Stem Cells (hBMMSCs) Maintain POSTN Secretion to Enhance the Migration Capability of Allogeneic hBMMSCs through the Integrin αvβ3/FAK/ERK Signaling Pathway

Figure 5

Migration capability of allo-hBMMSCs was enhanced by the culture supernatant of CTLA4- and POSTN-modified hBMMSCs cocultured with PBMCs treated with PHA. The concentrated culture supernatant from NC hBMMSCs, CTLA4-modified hBMMSCs, POSTN-modified hBMMSCs, or CTLA4+shPOSTN-modified hBMMSCs cocultured with PBMCs treated with PHA was added into the culture medium of allo-hBMMSCs in a ratio of 1 : 10, and the treated allo-hBMMSCs were named as NC, CTLA4, POSTN, and CTLA4+shPOSTN, according to their treatment. The migration capability of allo-hBMMSCs in each group was evaluated by Transwell assay (a), wound healing assay (b), and western blot to detect MMP2 and MMP9 levels (c). For all panels, left is the representative graphs and right is the statistical results based on three independent experiments. , when compared to the NC group; ^#, when compared to the POSTN group.

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