hCTLA4-Gene-Modified Human Bone Marrow-Derived Mesenchymal Stem Cells (hBMMSCs) Maintain POSTN Secretion to Enhance the Migration Capability of Allogeneic hBMMSCs through the Integrin αvβ3/FAK/ERK Signaling Pathway

Figure 7

Integrin αvβ3/FAK/ERK signaling pathway was blocked by anti-integrin αvβ3 treatment. Approximately 2 μg/mL negative control IgG or anti-integrin αvβ3 was added into the culture medium of allo-hBMMSCs with the concentrated culture supernatant from CTLA4-modified hBMMSCs or POSTN-modified hBMMSCs and allo-hBMMSCs cocultured with PBMCs treated with PHA, and treated allo-hBMMSCs were named CTLA4+IgG, CTLA4+anti-integrin αvβ3, POSTN+IgG, or POSTN+anti-integrin αvβ3. Protein levels of key factors in the integrin αvβ3/FAK/ERK signaling pathway were detected by western blotting. (a) Representative graphs of western blotting and (b) statistical results of relative protein expression based on three independent experiments. , when compared to the IgG group.

(a)

(b)