Stem Cells International / 2020 / Article / Fig 3

Research Article

The Exposure to Osteoarthritic Synovial Fluid Enhances the Immunomodulatory Profile of Adipose Mesenchymal Stem Cell Secretome

Figure 3

The conditioned medium of SF-exposed AMSCs inhibits differentiation of monocytes into DCs. Monocytes (M0) were differentiated into dendritic cells (DCs) in the presence of GM-CSF/IL-4 and LPS. To characterize dendritic cells, the expression of CD14 ((a)1), CD83 ((b)1), CD123 ((c)1), HLA-DR ((d)1), and CD80 ((e)1) was evaluated by flow cytometry. Conditioned medium of unstimulated (CM) or SF-exposed AMSCs (CM+SF) or synovial fluid only (SF) was added during DC differentiation. The expression levels of dendritic cells’ markers are presented as fold increase compared to untreated mDCs ((a)2, (b)2, (c)2, (d)2, and (e)2). The secretion of IL-10 in mDCs treated with CM, CM+SF, and SF is expressed as fold increase with respect to the secretion of IL-10 in untreated mDCs. Data are shown as Difference with untreated cells (). Difference with untreated cells (). Difference with untreated cells ().

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