Multimodal Therapeutic Effects of Neural Precursor Cells Derived from Human-Induced Pluripotent Stem Cells through Episomal Plasmid-Based Reprogramming in a Rodent Model of Ischemic Stroke

Figure 7

Glial scar formation and apoptosis analyses in MCAo rats with ep-iPSC-NPC transplantation. (a) Immunofluorescence staining of glial fibrillary acidic protein (GFAP) in the brains of rats in the ep-iPSC-NPC, fibroblast, and vehicle groups ( in each group). LV indicates the lateral ventricle. (b) Immunofluorescence staining of TUNEL (upper panel) and caspase-3 (lower panel) in the brains of rats of the ep-iPSC-NPC, fibroblast, and vehicle groups ( in each group). All samples were counterstained with DAPI (blue). Scale bars: 50 μm. Data were shown as (SEM), by one-way ANOVA. ep-iPSC-NPCs: neural precursor cells differentiated from induced pluripotent stem cells; MCAo: middle cerebral artery occlusion; TUNEL: terminal deoxynucleotidyl transferase-mediated dUTP nick-end labeling.

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