Human Adipose Derived Cells in Two- and Three-Dimensional Cultures: Functional Validation of an In Vitro Fat Construct

Figure 6

ObaGel cultures exhibit physiologically relevant functionality. Adiponectin (AdN) and leptin (Lep) secretions were measured from traditional cell cultures (2D) compared to three-dimensional adipose cultures (ObaCell) after 21 days of culture. Values reported are in pg/mL conditioned medium and are reported as (SD). . Adipose tissue functionality demonstrated with measurement of glycerol secretion in a conditioned medium from cells differentiated in two dimensions (2D) compared to ObaGel after 14 days of culture. ObaGel cultures demonstrated higher sensitivity to AMPK-targeting compounds isoproterenol, metformin, compound C, and IBMX. Values reported are in pg/mL conditioned medium and are reported as (SD). .

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