Antibiotics Disturb Dentin Formation and Differentiation of Dental Pulp Stem Cells: The Role of Microbiota in Cellular Turnover of Mouse Incisor

Figure 4

SCFAs but not LPS could rescue the mouse incisor growth rate and reinforced characteristics of DPSCs after antibiotic treatment. (a) Experimental procedure schematic. (b) The result of recovered height of injured incisors at the first day showed significant reduction in AbT compared to SPF and SCFA gavage, while LPS gavage showed no difference when compared with AbT. (c) DPSCs derived from SCFA mice showed greater colony-forming and proliferative capacity in vitro. (d) Alizarin red staining showed that SCFA-DPSCs formed more mineralized nodule than AbT DPSCs, and there was no significant difference between SCFA and SPF. (e) Real-time PCR results showed that the expression of DSPP, ALP, and BSP was higher in SCFA compared to that of AbT, while SPF showed the highest expression of mRNA above. All experimental data were verified in at least three independent experiments. Error bars represent the SEM from the mean values. ; ; . AbT: antibiotic treated; ConvD: conventionalized; SPF: specific pathogen-free; DSPP: dentin sialophosphoprotein; ALP: alkaline phosphatase; BSP: bone sialoprotein.

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