Mechanical Strain-Mediated Tenogenic Differentiation of Mesenchymal Stromal Cells Is Regulated through Epithelial Sodium Channels

Figure 2

The expression of the α-, β-, γ-, and δ-ENaC mRNA in unstrained and strained hMSCs. (a) Analysis of RT-PCR products from hMSCs indicating the presence of the ENaC subunits. (b) mRNA expression of hMSCs shows α-ENaC expressed highest in 8% strained compared with 4% and 12% strained. (c) Expression of ENaC subunits at 8% strained at 1 Hz. Fold changes of expression were counted by normalizing to the relative expression amount of corresponding control groups (unstrained groups). Statistical significance () was represented by which compared to unstrained. Error bars represent the SD of the mean of six biological replicates.

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