Research Article

Three Growth Factors Induce Proliferation and Differentiation of Neural Precursor Cells In Vitro and Support Cell-Transplantation after Spinal Cord Injury In Vivo

Figure 3

Differentiation of NPCs towards the oligodendroglial lineage in vitro. (a-c) Costaining of GFP+/NG2+ cells (blue arrows) indicating oligodendrocyte precursors. (d) Quantification of oligodendrocyte precursors after 7 days of incubation with the different growth factor concentrations/combinations in groups 2-4, expressed in proportion to (fold to) the control group (group 1). (e-g) Costaining of GFP+/APC+ cells (blue arrows) indicating mature oligodendrocytes. (h) Quantification of mature oligodendrocytes after 7 days of incubation with the different growth factor concentrations/combinations in groups 2-4, expressed in proportion to (fold to) the control group (group 1).