Three Growth Factors Induce Proliferation and Differentiation of Neural Precursor Cells In Vitro and Support Cell-Transplantation after Spinal Cord Injury In Vivo

Figure 4

Differentiation of NPCs into astrocytes and quantification of undifferentiated NPCs in vitro. (a-c) Costaining of GFP⁺/GFAP⁺ cells (blue arrows) indicating astrocytes. (d) Quantification of astrocytes after 7 days of incubation with the different growth factor concentrations/combinations in groups 2-4, expressed in proportion to (fold to) the control group (group 1). (e-g) Costaining of GFP⁺/Nestin⁺ cells (blue arrows) indicating undifferentiated NPCs. (h) Quantification of undifferentiated NPCs after 7 days of incubation with the different growth factor concentrations/combinations in groups 2-4, expressed in proportion to (fold to) the control group (group 1).