Essentiality of CTNNB1 in Malignant Transformation of Human Embryonic Stem Cells under Long-Term Suboptimal Conditions

Figure 1

CTNNB1 expression is upregulated during the malignant transformation of hESCs. MS/MS spectra of iTRAQ-labeled peptides from CTNNB1. (a) The relative peak heights of iTRAQ labels. (b) A representative tandem mass spectrum of a peptide in each protein. Reporter ions in (a) demonstrate examples of equal, low, and high protein levels in normal and aberrant chHES-3 cells. Normal, SIMP, COMP, and NTERA-2 cells are labeled as 114, 115, 116, and 117, respectively. The -axis of a mass spectrum represents the correlation between the mass of a given ion and the number of elementary charges. This is represented as the IUPAC standard m/z to denote the quantity generated by dividing the mass of an ion by the unified atomic mass unit and its charge number (positive absolute value). Data are represented in Dalton (Da) as the unit of mass. The -axis of a mass spectrum represents the signal intensity of ions.