Size-Optimized Microspace Culture Facilitates Differentiation of Mouse Induced Pluripotent Stem Cells into Osteoid-Rich Bone Constructs

Figure 1

Microspace size influences the condensation of iPSC aggregates. (a) Fabrication and osteogenic induction of 3D-iPSC constructs. Representative images of iPSC aggregates were obtained under phrase-contrast microscopy after 2 days of aggregation in Elp400, Elp500, and Elp900. Scale bars: 200 μm. (b) Size measurement of iPSC aggregates using ImageJ software (National Institutes of Health, Bethesda, MD, USA) to analyze Ferret’s diameter at culture days 2 and 5. Different letters indicate significant differences between each group (, ANOVA with Tukey’s multiple comparison test). The data represent the (). (c) Cell-cell adhesion marker gene expression with different microspace sizes. E-cadherin expression was determined by quantitative real-time RT-PCR at days 2 and 5. Different letters indicate significant differences between groups (, ANOVA with Tukey’s multiple comparison test). The data represent the (). (d) Representative images of nuclear staining of iPSC aggregates using Hoechst. Scale bars: 200 μm.

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