Intrathecal Infusion of Autologous Adipose-Derived Regenerative Cells in Autoimmune Refractory Epilepsy: Evaluation of Safety and Efficacy
ADRC in vitro analysis. (a) Flow cytometry analysis—surface markers analysis of ADRC. (b) Quantification of CFU frequency—P3: responder, P4: mild responder, P6: nonresponder, t2: ADRC isolated after 2nd liposuction, t3: ADRC isolated after 3rd liposuction. (c) CFU morphology from P3: (A) paraclone; (B) meroclone-like structures, scale bar—500 μM. (d) The morphology of ADRC derived from P3, scale bar—100 μM. (e) Secretory properties of ADRC exposed to patients CSF: Luminex multiplex cytokine analysis method. Dark grey bars: control, 48 h after incubation with CSF aspirated from responder (P3), mild responder (P6), and nonresponder (P4) before therapy.