Surgical Injury and Ischemia Prime the Adipose Stromal Vascular Fraction and Increase Angiogenic Capacity in a Mouse Limb Ischemia Model

Figure 4

Change in cellular compositions of SVFs by priming. Flow cytometry analysis of surface antigens in primed (injury+ischemia) and nonprimed SVFs. (a) CD45-positive mononuclear (left) and CD31-CD45-double negative (right) mesenchymal cell fractions on days 1, 3, and 7 after priming. (b) Scatterplots of CD45-positive and CD31-CD45-double negative cells are shown. Note that the primed and nonprimed SVFs were clearly segregated on day 7 after priming. (c) Mesenchymal-gated CD34-positive adipose progenitors, mesenchymal-gated CD140A-positive/CD9-high preadipocytes, or mesenchymal-gated CD140A-positive/CD9-low prefibroblasts on days 1, 3, and 7 after priming. (d) F4/80-positive macrophages, F4/80-positive/CD11c-positive/CD206-negative M1 macrophages, and F4/80-positive/CD11c-negative/CD206-positive M2 macrophages on days 1, 3, and 7 after priming. ; , , and primed vs. nonprimed SVFs. SVF: stromal vascular fraction.

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