Phosphodiesterase 10A Is a Mediator of Osteogenic Differentiation and Mechanotransduction in Bone Marrow-Derived Mesenchymal Stromal Cells

Figure 4

Analysis of osteogenic differentiation in primary human MSC (). (a) QPCR analyses of SPP1, RUNX2, and ALPL expression after 14 days of osteogenic differentiation and respective controls with and without 10 μM papaverine or 1 mM db-cAMP. RPS27A was used as the housekeeping gene. QPCR data were obtained from technical triplicates and results are shown as . Fold change was calculated with the ΔΔCT method. Significances were calculated with the Student -test (; ). (b) Alizarin red staining of primary MSC differentiated towards the osteogenic lineage for 14 days with and without application of 10 μM papaverine or 1 mM db-cAMP. Four representative donors are shown. The bar represents 100 μm. ALPL: tissue nonspecific alkaline phosphatase; c: control; db-cAMP: dibutyryl-cAMP; oD: osteogenic differentiation; Papa: papaverine; RUNX2: runt-related transcription factor 2; SPP1: secreted phosphoprotein 1.

(a)

(b)