Extensive Characterization of Mesenchymal Stem Cell Marker Expression on Freshly Isolated and In Vitro Expanded Human Adipose-Derived Stem Cells from Breast Cancer Patients

Figure 6

Representative images of multilineage differentiation ability of the expanded ADSC at passage 3. Cell histology in 2-dimentional culture of the induced cells was compared to that of the noninduced cells after different induction conditions: (a) adipogenic differentiation for 14 days and staining with Oil Red O; (b) osteogenic differentiation for 14 days and staining with Alizarin Red S; and (c) chondrogenic differentiation for 21 days and staining with Alcian Blue. All samples were observed using the inverted fluorescent microscope (10x magnification, scale bars: 100 μm).