Hypoxia-Induced Mesenchymal Stem Cells Exhibit Stronger Tenogenic Differentiation Capacities and Promote Patellar Tendon Repair in Rabbits

Figure 5

The mRNA and protein expressions of tenogenic markers in AMSCs and BMSCs at 7 days after induction. (a–d) qRT-PCR analysis of gene expression of Col-1a1 (a), Col-3a1 (b), Dcn (c), and Tnmd(d) under normoxia, Tgf-β1, hypoxia, and hypoxia+Tgf-β1 condition in AMSCs and BMSCs. (e–i) Representative western blots (e) and quantification of protein expression of Col-1a1 (f), Col-3a1 (g), Dcn (h), and Tnmd(i) under normoxia, Tgf-β1, hypoxia, and hypoxia+Tgf-β1 condition in AMSCs and BMSCs. Asterisks in the column in BMSCs indicated significant difference from the same condition in AMSCs. (j, k) Representative immunofluorescence staining (j) of Tnmd (red) and DAPI-labeled nuclei (blue), and quantification data (k) under under normoxia, Tgf-β1, hypoxia, and hypoxia+Tgf-β1 condition in AMSCs and BMSCs. Asterisks in the column in BMSCs indicated significant difference from the same condition in AMSCs. μm; Magnification: ×400. Data were shown as . ^★; ^★★.

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