A Xeno-Free Strategy for Derivation of Human Umbilical Vein Endothelial Cells and Wharton’s Jelly Derived Mesenchymal Stromal Cells: A Feasibility Study toward Personal Cell and Vascular Based Therapy
Purification of HUVECs and WJ-MSCs obtained by using a single umbilical cord with xeno-free cell isolation and expansion strategy. (a) Representative flow cytometry analysis of expanded HUVECs and WJ-MSCs stained with antibodies directed against human antigens specific for pan leukocyte (CD45), ECs (CD31), and MSCs: CD105, CD73, and CD90. (b) Percentage of HUVECs (light bar) and WJ-MSC-s (gray bar) stained positive for endothelial and MSC markers. Data were expressed as ().