Susceptibility to Ventricular Arrhythmias Resulting from Mutations in FKBP1B, PXDNL, and SCN9A Evaluated in hiPSC Cardiomyocytes
RT-qPCR analysis of SCN9A and SCN5A in hiPSC-CMs (normalized to GAPDH). SCN5A message was highly expressed, whereas levels of SCN9A were negligible in the hiPSC-CMs (a). RT-qPCR analysis of SCN9A and SCN5A in TSA201 cells transfected with SCN5A and SCN9A plasmid at a 1 : 1 molar ratio. SCN9A message could readily be detected in transfected TSA201 cells (b). RT-qPCR analysis of PXDN and PXDNL in hiPSC-CMs (c). PXDNL was expressed at low levels in both WT and MMRL1126 hiPSC-CMs.