Myocardin-Related Transcription Factor A (MRTF-A) Regulates the Balance between Adipogenesis and Osteogenesis of Human Adipose Stem Cells

Figure 3

Early osteogenesis of MRTF-A inhibitor-treated hASCs. The hASCs were cultured in BM or OM supplemented with CCG-1423 (a) or CCG-100602 (b) inhibitors in addition to medium controls. ALP activity was analyzed with ALP assay at 7 d and 14 d. The ALP absorbance values were normalized with corresponding CyQUANT results, and the results are presented relative to the 7 d BM sample. , independent biological replicates from 4 donors. Significance level 5%. (c) Representative images of COL-1 (Alexa 488, green)-, actin (Alexa 546, red)-, and nuclei (DAPI, blue)-stained hASCs at 14 d, after culture with 20 μM CCG-1423 or 12 μM CCG-100602. Images with 10x and 40x magnifications are provided to give an overall view and a more detailed view of intracellular localization of COL-1. Scale bars 100 μm. BM: basic medium; OM: osteogenic medium.

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