Research Article

Histone Demethylase KDM4C Is Required for Ovarian Cancer Stem Cell Maintenance

Figure 4

Increased KDM4C occupancy and H3K9 methylation levels at the OCT-4 gene promoter. (a) ChIP analysis of KDM4C occupancy at the OCT-4 promoter after KDM4C inhibition as quantified by real-time PCR. (b) ChIP analysis of H3K9 dimethylation levels at the OCT-4 promoter. (c) ChIP analysis of H3K9 trimethylation levels at the OCT-4 promoter as quantified by real-time PCR. (d) Western Blot analysis of SK-OV-3 spheres for KDM4C and OCT-4 expression. α-Tubulin is used as the control. .