Regulation of CXCR6 Expression on Adipocytes and Osteoblasts Differentiated from Human Adipose Tissue-Derived Mesenchymal Stem Cells

Figure 6

CXCL16 cleavage by ADAM10 activation in hADMSCs and differentiated adipocytes or osteoblasts. (a) Expression of transmembrane-bound CXCL16 on the cell surface was evaluated by ICC. Cells were stained with CXCL16 (green), and nuclei were stained with DAPI (blue). . (b) Normalized data obtained by using the ImageJ program. trial per samples and control. (c) Level of soluble CXCL16 in the cell supernatant of hADMSCs, Ad (12 days), Ad (18 days), Os (12 days), and Os (18 days) was examined by ELISA. trial per samples and control. (d) Protein expression of pro- and mature form of ADAM10 and ADAM17. p: proform; m: mature form. Quantification of pro- or mature form of (e) ADAM10 and ADAM17. trial per samples and control. , , and indicate statistically significant compared with the hADMSC group. ns: not significant.

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