Small Extracellular Vesicles from Human Fetal Dermal Cells and Their MicroRNA Cargo: KEGG Signaling Pathways Associated with Angiogenesis and Wound Healing

Table 1

EV-derived miRNAs found highly expressed in fetal samples (), which were also upregulated compared to adult samples (see also Supplemental Material S1).

miRNA name	Role in angiogenesis/tissue regeneration	References	Fold increase in fetal vs. adult samples	value
hsa-let-7b-5p	It targets VEGF gene; validated role in angiogenesis	Hua et al., 2006 [36]; Landskroner-Eiger et al., 2013 [34]	26.2091	0.0074
hsa-let-7g-5p	Validated role in angiogenesis	Landskroner-Eiger et al., 2013 [34]	175.3585	0.021
hsa-miR-10a-5p	Validated role in angiogenesis; angiogenesis influencer	Landskroner-Eiger et al., 2013 [34]	242.9505	0.03
hsa-miR-10b-3p			17.9198	0.0346
hsa-miR-15b-5p	Angiogenesis regulator; delivered in regenerative medicine for cardiac repair	Curtin et al., 2018 [29]; Wang & Olson, 2009 [39]	2624.268	0.0003
hsa-miR-16-5p	Angiogenesis regulator; it targets VEGF gene; regulator of angiogenesis; induces tube formation of HUVECs	Hua et al., 2006 [36]; Poliseno et al., 2006 [78]; Suarez & Sessa, 2009 [38]; Wang & Olson, 2009 [39]	149.6445	0.0183
hsa-miR-17-5p	Angiogenesis regulator; it targets VEGF gene	Caporali & Emanueli, 2011 [33]; Hua et al., 2006 [36], Wang & Olson, 2009 [39]	133.7475	0.0032
hsa-miR-19a-3p	Validated role in angiogenesis	Landskroner-Eiger et al., 2013 [34]; Wang & Olson, 2009 [39]	51.8807	0.0114
hsa-miR-19b-3p	Angiogenesis regulator	Caporali & Emanueli, 2011 [33]; Wang & Olson, 2009 [39]	109.3557	0.0044
hsa-miR-20a-5p	It targets VEGF gene; validated role in angiogenesis	Hua et al., 2006 [36]; Landskroner-Eiger et al., 2013 [34]; Wang & Olson, 2009 [39]	56.7629	0.0004
hsa-miR-21-5p	Angiogenesis regulator; wound healing regulator	Wang & Olson, 2009 [39]; Wang et al., 2012 [32]	157.6826	0.0046
hsa-miR-24-3p	Highly expressed by endothelial cells	Suarez & Sessa, 2009 [38]; Zhou et al., 2011 [40]	25.4984	0.0059
hsa-miR-26a-5p	Proregenerative (it promotes osteogenesis-angiogenesis in mouse)	Li et al., 2013 [47]	132.6638	0.0122
hsa-miR-26b-5p	Tissue repair; remodeling in wound healing	Banerjee & Sen, 2013 [31]; Sen et al., 2015 [30]	95.4933	0.0183
hsa-miR-27b-3p	It targets VEGF gene; validated role in angiogenesis	Hua et al., 2006 [36]; Landskroner-Eiger et al., 2013 [34]; Wang & Olson, 2009 [39]; Zhou et al., 2011 [40]	950.021	0.0096
hsa-miR-28-3p			49.9995	0.0082
hsa-miR-29b-3p	Regulator of tissue regeneration; proregenerative (delivered for ECM remodeling in fibrosis treatment)	Monaghan et al., 2014 [42]; van Rooij et al., 2008 [43]	61.9175	0.0105
hsa-miR-30a-3p	It targets VEGF gene; endothelial cell modulator	Bridge et al., 2012 [35]; Hua et al., 2006 [36]	185.51	0.0099
hsa-miR-30b-5p	Angiogenesis regulator; endothelial cell modulators; it targets VEGF gene	Bridge et al., 2012 [35]; Hua et al., 2006 [36]	32.3336	0.0004
hsa-miR-30c-5p	Endothelial cell modulator	Bridge et al., 2012 [35]	30.1373	0.0002
hsa-miR-30e-3p	Endothelial cell modulator	Bridge et al., 2012 [35]	41.2049	0.0041
hsa-miR-31-5p	Angiogenesis regulator; wound healing	Li et al., 2015 [79]; Wang et al., 2012 [32]	60.803	0.0027
hsa-miR-31-3p	Angiogenesis regulator; wound healing	Li et al., 2015 [79]; Wang et al., 2012 [32]	229.5474	0.0097
hsa-miR-34a-5p	It targets VEGF gene		2254.5936	0
hsa-miR-92a-3p	Validated role in angiogenesis	Curtin et al., 2018 [29]; Landskroner-Eiger et al., 2013 [34]; Wang & Olson, 2009 [39]	32.781	0.0001
hsa-miR-93-3p			149.3753	0.0065
hsa-miR-99b-3p	Angiogenesis promoter	Kane et al., 2012 [37]	37.2967	0.0134
hsa-miR-99b-5p	Angiogenesis promoter	Kane et al., 2012 [37]	41.1216	0.0031
hsa-miR-103a-3p			809.2304	0.008
hsa-miR-106a-5p	It targets VEGF gene; regulator of angiogenesis	Hua et al., 2006 [36]	67.8178	0.0047
hsa-miR-106b-5p	It targets VEGF gene; regulator of angiogenesis	Hua et al., 2006 [36]; Landskroner-Eiger et al., 2013 [34]	56.2099	0.0084
hsa-miR-125a-5p	Tube formation of HUVECs; it targets VEGF gene	Hua et al., 2006 [36]; Poliseno et al., 2006 [78]	128.8981	0.0061
hsa-miR-125b-5p	Angiogenesis regulator; tube formation of HUVECs	Poliseno et al., 2006 [78]; Zhou et al., 2015 [80]	18.6552	0.0041
hsa-miR-127-3p			19.2386	0.0001
hsa-miR-132-3p	Validated role in angiogenesis during chronic wound healing; proregenerative (it promotes angiogenesis in myocardial infarction)	Landskroner-Eiger et al., 2013 [34]; Li et al., 2017 [81]; Ma et al., 2018 [44]	3664.7066	0.0034
hsa-miR-136-3p			164.0836	0.0268
hsa-miR-138-5p			52.6792	0.0162
hsa-miR-145-5p	Angiogenesis regulator	Fan et al., 2012 [82]	42.2841	0.0067
hsa-miR-146a-3p	Wound healing (inflammatory phase)	Banerjee & Sen, 2013 [31]	172.0832	0.0135
hsa-miR-146b-3p	Wound healing (inflammatory phase); angiogenesis promoter	Ahn et al., 2013 [83]; Banerjee & Sen, 2013 [31]	203.771	0.0161
hsa-miR-149-5p	Scarless wound healing	Lang et al., 2017 [84]	37.5122	0.0102
hsa-miR-151a-5p			126.8167	0.005
hsa-miR-151a-3p			17.732	0.0246
hsa-miR-152-3p			20.0452	0.0097
hsa-miR-155-5p	Wound healing (inflammatory phase); amyotrophic lateral sclerosis; anti-inflammatory action; angiogenesis regulator	Banerjee & Sen, 2015 [31]; Curtin et al., 2018 [29]; Suarez & Sessa, 2009 [38]	32.5772	0.04
hsa-miR-181a-5p	Angiogenesis promoter	Kane et al., 2012 [37]	112.4827	0.0011
hsa-miR-186-5p			78.0074	0.0165
hsa-miR-191-5p	It may regulate the angiogenic actions of VEGF	Landskroner-Eiger et al., 2013 [34]	66.0569	0.0121
hsa-miR-193a-5p	It targets VEGF gene	Hua et al., 2006 [36]	108.2673	0.0061
hsa-miR-193b-3p	Proregenerative (chondrogenesis)	Meng et al., 2018 [45]	16.6788	0.0057
hsa-miR-197-3p			47.9232	0.01
hsa-miR-199a-3p	It targets VEGF gene; proregenerative (cardiac regeneration)	Hua et al., 2006 [36]; Lesizza et al., 2017 [46]	79.9505	0.0112
hsa-miR-214-3p			34.8695	0.0131
hsa-miR-214-5p	It targets VEGF gene	Hua et al., 2006 [36]	493.6614	0.0151
hsa-miR-218-5p	Validated role in angiogenesis	Landskroner-Eiger et al., 2013 [34]	32.9227	0.0092
hsa-miR-221-3p	Validated role in angiogenesis	Landskroner-Eiger et al., 2013 [34]	27.5391	0.0003
hsa-miR-222-3p	Angiogenesis in wound healing; validated role in angiogenesis	Banerjee & Sen, 2015 [31]; Landskroner-Eiger et al., 2013 [34]	38.7974	0.0103
hsa-miR-224-5p			21.5539	0.0039
hsa-miR-320a	It targets VEGF gene; validated role in angiogenesis	Hua et al., 2006 [36]; Landskroner-Eiger et al., 2013 [34]	56.2268	0.0086
hsa-miR-323a-3p			686.8069	0.0163
hsa-miR-324-3p			137.7004	0.0144
hsa-miR-331-3p	It targets VEGF gene	Hua et al., 2006 [36]	43.6136	0.0043
hsa-miR-342-3p			169.8914	0.0114
hsa-miR-345-5p			200.3964	0.0134
hsa-miR-365a-3p			526.7213	0.0115
hsa-miR-370-3p			99.3169	0.0081
hsa-miR-374a-5p			44.7501	0.0225
hsa-miR-376a-3p			216.5826	0.0246
hsa-miR-376c-3p			216.5826	0.0246
hsa-miR-382-5p			6102.4887	0.0009
hsa-miR-409-3p			173.9373	0.0097
hsa-miR-411-5p			99.6678	0.0353
hsa-miR-424-3p			482.6326	0.0137
hsa-miR-432-5p			125.5471	0.0385
hsa-miR-433-3p			778.7489	0.0057
hsa-miR-455-5p			611.2326	0.0122
hsa-miR-484			85.625	0.0066
hsa-miR-487b-3p			131.5413	0.0028
hsa-miR-493-3p			1303.6278	0.0053
hsa-miR-532-3p			42.572	0.0015
hsa-miR-532-5p	Angiogenesis	Slater et al., 2018 [85]	1363.2918	0.0072
hsa-miR-539-5p			350.9595	0.0063
hsa-miR-574-3p			59.5134	0.0121
hsa-miR-625-3p			77.7476	0.002
hsa-miR-708-5p			59.2604	0.0013
hsa-miR-766-3p			1533.4818	0.016
hsa-miR-886-5p			75.0277	0.0048
hsa-miR-1290			24.3418	0.0021

Plotted values () represent fetal samples () compared to adult samples ().