Research Article
Isolation and Characterization of Multipotent Canine Urine-Derived Stem Cells
Table 2
Reagents and formula of proliferation medium for canine USCs.
| | Reagents | Formula (mL) |
| | DMEM/F-12 (Gibco, USA) | 43.5 mL | | RE basal medium (Lonza, USA) | 42.9 mL | | REGM SingleQuot kit (Lonza, USA) | 600.0 μL | | Fetal bovine serum (Gibco, USA) | 10.0 mL | | Antibiotic-antimycotic (Gibco, USA) | 1.0 mL | | GlutaMAX (Gibco, USA) | 1.0 mL | | MEM non-essential amino acids (Gibco, USA) | 1.0 mL | | bFGF (Peprotech, USA) | 500 ng | | PDGF-BB (Peprotech, USA) | 500 ng | | EGF (Peprotech, USA) | 500 ng |
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