|
| Markers | Gene | Primer sequence (5-3) |
|
| Osteogenic genes | Runx2 | Forward CAGACCAGCAGCACTCCATA |
| Reverse CAGCGTCAACACCATCATTC |
| Bglap | Forward CTGAATCCCGCAAAGGTGGT |
| Reverse CTCGTCACAGTTGGGGTTGA |
| Spp1 | Forward TAGCCAGGACTCCGTTGACT |
| Reverse ACACTATCACCTCGGCCATC |
|
| Chondrogenic genes | Sox9 | Forward GCTCGCAGTACGACTACACTGAC |
| Reverse GTTCATGTAGGTGAAGGTGGAG |
| Col2a1 | Forward GAAACTCTGCCACCCTGAATG |
| Reverse GCTCCACCAGTTCTTCTTGG |
| Acan | Forward ATCAACAGTGCTTACCAAGACA |
| Reverse ATAACCTCACAGCGATAGATCC |
|
| Adipogenic genes | PPARγ | Forward primer TCACAGAGTACGCCAAAAGT |
| Reverse primer ACTCCCTTGTCATGAATCCT |
| FABP4 | Forward ATCAGTGTAAACGGGGATGTG |
| Reverse GACTTTTCTGTCATCCGCAGTA |
| LPL | Forward ACACATTCACAAGAGGGTCAC |
| Reverse CTCTGCAATCACACGGATG |
|
| Tenogenic genes | Tnmd | Forward GATCCCATGCTGGATGAG |
| Reverse TACAAGGCATGATGACACG |
| Scx | Forward AAGCTCTCCAAGATCCGAGACACTG |
| Reverse AAGAAGGGCCCAGAGTGGC |
| Mkx | Forward AGACATGTCATGGCCACAAA |
| Reverse TGATGATGAGGGAGACACCA |
|
| Housekeeping | GAPDH | Forward CCATCTTCCAGGAGCGAGAT |
| Reverse TTCTCCATGGTGGTGAAGAC |
|
