Differentiation Potential of Early- and Late-Passage Adipose-Derived Mesenchymal Stem Cells Cultured under Hypoxia and Normoxia

Table 1

The information of the specific genes.

Gene marker type	Name of gene primer	Gene name	Accession number	Forward and reverse sequences	PCR product length (bps)
Housekeeping	Glyceraldehyde-3-phosphate dehydrogenase	GAPDH	NM_002046.5	F: ATGTTCGTCATGGGTGTGAA; R: TGTGGTCATGAGTCCTTCCA	144
Pluripotent gene	POU class 5 homeobox	POU5F1	NM_002701.5	F: GCAATTTGCCAAGCTCCTGAA; R: AGCTAAGCTGCAGAGCCTCAAAG	141
	Nanog homeobox	NONAG	NM_024865.3	F: CAACTGGCCGAAGAATAGCAATG; R: TGGTTGCTCCAGGTTGAATTGTT	159
	Kruppel-like factor 4	KLF4	NM_004235.5	F: AAGAGTTCCCATCTCAAGGCACA; R: GGGCGAATTTCCATCCACAG	91
Osteogenic gene	Runt-related transcription factor 2	RUNX2	NM_004348.3	F: ATGTGTTTGTTTCAGCAGCA; R: TCCCTAAAGTCACTCGGTATGTGTA	195
	Collagen type I alpha 1 chain	COL1A1	NM_000088	F: GCTACCCAACTTGCCTTCATG; R: TGCAGTGGTAGGTGATGTTCTGA	168
Chondrogenic gene	SRY-box 9	SOX9	NM_000346.3	F: TGTATCACTGAGTCATTTGCAGTGT; R: AAGGTCTGTCAGTGGGCTGAT	187
	Collagen type II alpha 1 chain	COL2A1	NM_001844.4	F: TGAAGGTTTTCTGCAACATGGA; R: TTGGGAACGTTTGCTGGATT	67
Adipogenic gene	Peroxisome proliferator-activated receptor gamma	PPARG	NM_005037.5	F: TGGAATTAGATGACAGCGACTTGG; R: CTGGAGCAGCTTGGCAAACA	182