Research Article
MEST Regulates the Stemness of Human Periodontal Ligament Stem Cells
Table 1
Primer sequences, annealing temperatures, cycle numbers, and product sizes for semiquantitative RT-PCR.
| | Gene (abbreviation) | Primer sequence forward/reverse | Annealing temperature (°C) | Semiquantitative RT-PCR cycles | Size of amplified products (bp) |
| | COL-1 | 5-CCCGGGTTTCAGAGACAACTTC-3/5-TCCACATGCTTTATTCCAGCAATC-3 | 61 | 23 | 148 | | PLAP-1 | 5-ATGGGAGTCTTGCTAACATACCAC-3/5-CAGAAGTCATTTACTCCCACTCTTG-3 | 60 | 30 | 154 | | POSTN | 5-CACAACCTGGAGACTGGACA-3/5-TGCTTCCTTGTGTGGTCTTT-3 | 59 | 25 | 198 | | GAPDH | 5-ACCACAGTCCATGCCATCCAC-3/5-TCCACCACCCTGTTGCTGTA-3 | 60 | 20 | 452 |
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