Cloning and Embryo Splitting in Mammalians: Brief History, Methods, and Achievements

Table 5

Recent studies in embryo splitting.

Species	Embryo sample	Method	Results	Reference
Human	4-cell	Biopsy	NANOG expression in TE & ICM	[59]
Mouse	2-cell	Biopsy	Oct4 expression in blastocysts	[67]
Mouse	2-cell	Biopsy	Sox2 expression in blastocysts	[68]
Mouse	2-cell	Biopsy	Cdx2 expression in blastocysts	[69]
Mouse	2-cell	Biopsy	NANOG expression in blastocysts	[70]
Mouse	2-cell	Biopsy	100% Arntl and 91% Prrt2 knockout by C-CRISPR	[65]
Cynomolgus monkeys
Cynomolgus monkeys	2-cell	Biopsy	Dax1 knockout by CRISPR/Cas9	[71]
Rhesus monkey	4-cell	Biopsy	Dystrophin gene knockout by CRISPR/Cas9	[72]