Proteogenomic Analysis Reveals Proteins Involved in the First Step of Adipogenesis in Human Adipose-Derived Stem Cells

Figure 5

Gene expression regulation during the first step of human adipogenesis. (a) Scatterplot comparing RNA-seq from the total and polysomal fractions of hASC submitted to adipogenesis (24 hours), showing 53 proteins uniquely identified in 24 h-differentiated cells. (b) The same analysis with the 92 proteins uniquely identified in undifferentiated hASC. Orange and blue dots represent genes with . The named dots represent DEGs in the total and polysomal fractions. Orange and blue boxes represent adipogenesis (24 hours) and undifferentiated hASC, respectively. The differentially abundant proteins (up- and downregulated) were compared with the mRNA expression of the (c) total and (d) polysomal fraction. Orange and blue dots represent genes with in the ADI and CT conditions, respectively. Dashed red lines represent the cutoff criteria for fold change in the RNA-seq data.

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