Pluripotency-State-Dependent Role of Dax1 in Embryonic Stem Cells Self-Renewal

Figure 4

The global transcription profiles of Dax1KO cells in 2i/LIF and LIF/serum culture. (a) Scatterplots showing differentially expressed genes (DEGs) from Dax1 KO compared to wild-type ESCs cultured in 2i/LIF (left) and LIF/serum (right) medium. Grey dots are unaltered genes. Colored dots are significantly up-/downregulated genes in Dax1 KO cells with DESeq2 p (adj) ≤0.05, absolute value of fold change ≥1.2, the color of the dot correspond to the absolute value of fold change (red is high and blue is low). (b) Venn diagrams represent overlapped total DEGs (left), upregulated genes (middle), or downregulated genes (right) in Dax1 KO cells between 2i/LIF and LIF/serum medium. The red letters indicate the number of the DEGs with absolute fold change ≥2, and the black/white letters indicate the number of the DEGs with absolute fold change ≥1.2. (c) Enrichment map networks of GO terms corresponding to DEGs with absolute fold change ≥1.2 (blue) or DEGs with absolute fold change ≥2 (red) in Dax1 KO versus wild-type mESCs cultured in 2i/LIF (left) and LIF/serum (right) medium. Gene Ontology was analyzed by g: profiler, and visualized by the cytoscape plug-in: enrichment map. Nodes represent GO terms; edges connect nodes that share common genes.

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