Interleukin-20 Acts as a Promotor of Osteoclastogenesis and Orthodontic Tooth Movement

Figure 2

Overexpression of IL-20 regulates the expression of RANKL and OPG, indicating that IL-20 can modulate osteoclast differentiation through the OPG/RANKL/RANK axis. (a) Expression levels of IL-20 and its receptor IL-20RB in BMSCs and BMMs were determined by immunohistochemical staining. The use of an overexpression plasmid to enhance IL-20 expression in BMMs led to increased expression levels of RANKL and RANK, whereas it led to a decreased expression level of OPG. (b and c) BMMs were transfected with overexpression plasmids to increase the expression of IL-20, and the expression level of IL-20 was detected by qRT-PCR and western blotting; the control group received no plasmid. vs. control group. (d) mRNA expression levels of RANK, RANKL, and OPG were evaluated by qRT-PCR after 2 days of transfection. vs. control group; (e) protein expression levels of OPG, RANK, and RANKL were examined by western blotting. Bars represent the of three independent experiments (). vs. control group; N.S.: not significant.

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