|
| Methods | Advantages | Limitations |
|
| OP9/C3H10T1/2 feeder system [39] | The cornerstone of hPSC-MK generation | Low platelet production
Long induction period
Potential xenogenous contamination |
| ES-sac system [35] | Identifies most effective cytokines during hPSC-MK generation
The basis for efficient production of platelets | Long induction period
Potential xenogenous contamination
Low platelet production |
| EB formation system [40, 66] | Improves the efficiency of MK generationbased on the ES-sac system
Combined with defined serum- and animal feeder-free conditions
Provides evidence for the functionality of iPlatelets in vivo | Limited efficiency in platelet production |
| Feeder- or serum-free system [36] | Without pathogen contamination | Limited efficiency in platelet production |
| HLA-universal iPlatelets [38, 50, 67] | Shortens platelet production time
Increases MKP yield
Reduces the immunoreactivity of iPlatelets | Inevitable off-target effects or genome toxicity effects
Limited efficiency in platelet production |
| imMKCLs [44, 49] | High stability and cryopreserved storage
Widely used in future clinical applications
Combined with a bioreactor system | High cost
The potential risk of exogene integration |
| Other genetic manipulation [43] | Feasibility in genetic manipulation
Discovers new critical factors that determine the fate of iPlatelets | Inevitable off-target effects
Exogene integration may have some specific safety concerns in clinical treatment
Limited efficiency in platelet production |
|
